Coding

Part:BBa_K1179055:Design

Designed by: Brandon Nadres   Group: iGEM13_MIT   (2013-09-15)

VN173


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 205
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Venus fluorescent protein is a yellow fluorescent protein. The protein is spit at the 172nd residue. Therefore, VN173, consists of residues 1-172. No fluorescence is seen with transfection of a single split Venus protein.

Source

The venus fluorescent protein is a genetic mutant of GFP derived from Aequorea victoria. Sincere thanks to Samira Kiani for contributing this entry vector to the 2013 MIT iGEM team.

References